PIPOX Antikörper (Biotin)

Produktnummer ABIN106701

Hersteller Produkt- Nr.: 200-106-093-0100

Der Ziege Polyklonal Anti-PIPOX-Antikörper wurde für ELISA, WB und IP validiert. Er ist geeignet, PIPOX in Proben von Microbial zu detektieren.

Kurzübersicht für PIPOX Antikörper (Biotin) (ABIN106701)

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Reaktivität: Microbial

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PIPOX Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-PIPOX Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-106-093-0100

Hersteller: Rockland

Verwendungszweck: Sarcosine Oxidase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].

Produktmerkmale: Synonyms: goat anti-Sarcosine Oxidase Antibody, biotin Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit

Aufreinigung: Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Sarcosine Oxidase [Microbial]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Sarcosine Oxidase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 μg of Sarcosine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu PIPOX

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Andere Bezeichnung: Sarcosine Oxidase

Hintergrund: Background: Sarcosine Oxidase is an inducible enzyme that catalyzes the oxidative demethylation of sarcosine to glycine, hydrogen peroxide, and 5,10-methylene-tetrahydrofolate in the presence of tetrahydrofolate and oxygen. Sarcosine oxidase is also able to oxidize cyclic amino acids such as L-proline and L-pipecolic acid, albeit at reduced rates.

Gen-ID: 10586229

NCBI Accession: YP_004446819

UniProt: Q46337