RNASE1 Antikörper

Produktnummer ABIN106560

Hersteller Produkt- Nr.: 200-4188-0100

Dieses Anti-RNASE1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RNASE1 in WB, IP und ELISA. Geeignet für Rind (Kuh).

Kurzübersicht für RNASE1 Antikörper (ABIN106560)

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNASE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-RNASE1 Antikörper

Hersteller Produkt- Nr.: 200-4188-0100

Hersteller: Rockland

Verwendungszweck: Ribonuclease A Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas].

Produktmerkmale: Synonyms: rabbit anti-Ribonuclease A Antibody, Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

Aufreinigung: Anti-Ribonuclease A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ribonuclease A [Bovine pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Ribonuclease A Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Ribonuclease A [Bovine pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:40,000 to 1:180,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu RNASE1

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Andere Bezeichnung: RNASE1

Hintergrund: Background: Pancreatic ribonuclease is an endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA. This ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA, this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides. It has endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Gen-ID: 282340

UniProt: P61823