RPA2 Antikörper (pSer122)

Produktnummer ABIN106543

Hersteller Produkt- Nr.: 600-401-447

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RPA2 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae.

Kurzübersicht für RPA2 Antikörper (pSer122) (ABIN106543)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-RPA2 Antikörper

Bindungsspezifität: pSer122

Hersteller Produkt- Nr.: 600-401-447

Hersteller: Rockland

Verwendungszweck: RFA2 phospho S122 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against yeast RFA2.

Produktmerkmale: Synonyms: rabbit anti-RFA2 pS122 antibody, RFA-2, RFA 2, Replication factor A protein 2, RF-A protein 2, Replication protein A 36 kDa subunit, DNA-binding protein BUF1, BUF-1, YNL312W, N0368

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G₁/S transition and is dephosphorylated at mitosis.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity-purified antibody was tested for use in western blotting against RFA2 containing cell lysates and by ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:1000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:8,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.54 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RFA2

Hintergrund: Background: RFA2 binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). It stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

Gen-ID: 855404, 132475

UniProt: P26754

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA