Protein A Antikörper (Biotin)

Produktnummer ABIN106172

Hersteller Produkt- Nr.: 200-1677

Der Ziege Polyklonal Anti-Protein A-Antikörper wurde für ELISA, WB und IHC validiert. Er ist geeignet, Protein A in Proben von Staphylococcus aureus zu detektieren.

Kurzübersicht für Protein A Antikörper (Biotin) (ABIN106172)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Protein A Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-1677

Hersteller: Rockland

Verwendungszweck: Protein A Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].

Produktmerkmale: Synonyms: goat anti-Protein A Antibody biotin conjugated, goat anti Protein A biotin, goat anti PA biotin

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This product has been assayed against 1.0 μg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000-1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Protein A

Target: Protein A

Hintergrund: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.