Beta-Phosphoglucomutase Antikörper
Kurzübersicht für Beta-Phosphoglucomutase Antikörper (ABIN105570)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Hersteller Produkt- Nr.
- 200-101-237-0100
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Hersteller
- Rockland
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Verwendungszweck
- Beta-Phosphoglucomutase Antibody
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified b-Phosphoglucomutase [Lactococcus lacti].
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Produktmerkmale
- Synonyms: goat anti-Beta-Phosphoglucomutase Antibody, goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody
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Aufreinigung
- Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Immunogen
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Immunogen: Beta Phosphoglucomutase [Lactococcus lacti]
Immunogen Type: Native Protein
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Isotyp
- IgG
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anti-Phosphoglucomutase, beta- antibody (Biotin)
Reaktivität: Lactococcus lacti WB, ELISA Wirt: Ziege Polyclonal Biotin
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Applikationshinweise
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Application Note: Anti-Beta-Phosphoglucomutase Antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of b-Phosphoglucomutase [Lactococcus lacti] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Konzentration
- 10.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)
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Andere Bezeichnung
- pgmB
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Hintergrund
- Background: Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C6OH or C1OH, respectively.
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Gen-ID
- 1114041
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UniProt
- P71447
Target
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