Beta-Phosphoglucomutase Antikörper (Biotin)

Produktnummer ABIN1607648

Produktdetails anti-Beta-Phosphoglucomutase Antikörper (Biotin)

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Reaktivität: Lactococcus lacti

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Beta-Phosphoglucomutase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Beta-Phosphoglucomutase from other sources is unknown.

Reinheit: Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Beta-Phosphoglucomutase [Lactococcus lacti].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: beta Phosphoglucomutase [Lactococcus lacti]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Beta-Phosphoglucomutase has been assayed against 1.0 µg of Beta-Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:50.000 is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Beta-Phosphoglucomutase

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Andere Bezeichnung: Beta-Phosphoglucomutase (Beta-Phosphoglucomutase Produkte)

Synonyme: ECK1312 antikoerper, JW1310 antikoerper, beta-phosphoglucomutase antikoerper, ycjU antikoerper, pgmB antikoerper, LMOf2365_2822 antikoerper, Exig_2478 antikoerper, Hore_00430 antikoerper, Tmath_0800 antikoerper, Aazo_0214 antikoerper, Spirs_3639 antikoerper, Closa_0065 antikoerper, Toce_1769 antikoerper, Tthe_1054 antikoerper, Lbys_0589 antikoerper, Celal_0979 antikoerper, Sgly_0722 antikoerper, Celly_2426 antikoerper, Spico_1537 antikoerper, Theth_1551 antikoerper

Hintergrund: Anti-Beta-Phosphoglucomutase antibody is located in the cytoplasm, working to catalyze the transference of a phosphoryl group on a glucose or lactose monomer from the 1’ to the 6’ position in the forward direction or 6’ to the 1’ position in the reverse direction. Anti-Beta-Phosphoglucomutase is essential to regulating the flow of carbohydrate intermediates in glycolysis as well as the formation of the sugar nucleotide UDP-glucose. Part of the HAD-like hydrolase superfamily, Anti-Beta-Phosphoglucomutase antibody is ideal for researchers interested in Metabolism research. Synonyms: Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody

Gen-ID: 1114041

UniProt: P71447