ING4 Antikörper (AA 150-175)

Produktnummer ABIN105223

Hersteller Produkt- Nr.: 600-101-284

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ING4 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für ING4 Antikörper (AA 150-175) (ABIN105223)

Target: ING4 (Inhibitor of Growth Family, Member 4 (ING4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ING4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ING4 Antikörper

Bindungsspezifität: AA 150-175

Hersteller Produkt- Nr.: 600-101-284

Hersteller: Rockland

Verwendungszweck: p29ING4 Antibody

Kreuzreaktivität (Details): Reactivity occurs against human p29 ING4 protein.

Produktmerkmale: Synonyms: goat anti-p29 ING4 antibody, p29ING4, ING 4, ING-4, Inhibitor of growth family member 4 antibody, Inhibitor of growth family member 4 long isoform antibody, Inhibitor of growth protein 4 antibody, MGC12557 antibody

Aufreinigung: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity-purified antibody has been tested for use in ELISA against the immunizing peptide.  Specific conditions for western blotting reactivity should be optimized by the end user.  Expect a band at approximately 29 kDa in size corresponding to ING4 isoform 1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:3,000 - 1:12,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ING4 Antikörper (ABIN105223)

Colla, Tagliaferri, Morandi, Lunghi, Donofrio, Martorana, Mancini, Lazzaretti, Mazzera, Ravanetti, Bonomini, Ferrari, Miranda, Ladetto, Neri, Neri, Greco, Mangoni, Bonati, Rizzoli, Giuliani et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha ..." in: Blood, Vol. 110, Issue 13, pp. 4464-75, (2007) (PubMed).

Unoki, Shen, Zheng, Harris: "Novel splice variants of ING4 and their possible roles in the regulation of cell growth and motility." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34677-86, (2006) (PubMed).

Details zu ING4

Target: ING4 (Inhibitor of Growth Family, Member 4 (ING4))

Andere Bezeichnung: ING4

Hintergrund: Background: p29 ING4 is a tumor suppressor protein similar to ING1 that  may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA.  p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) that encodes the longest isoform.

Gen-ID: 51147

UniProt: Q9UNL4