VIPR1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049454

Der Kaninchen Polyklonal anti-VIPR1 Antikörper (ABIN1049454) detektiert spezifisch VIPR1 in IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für VIPR1 Antikörper (Cytoplasmic Domain) (ABIN1049454)

Target: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VIPR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-VIPR1 Antikörper

Bindungsspezifität: Cytoplasmic Domain

Spezifität: Human VIP Receptor 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GHRHR (56 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey, Marmoset, Rabbit (94%) Hamster, Pig (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human VIP Receptor 1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey, Marmoset, Rabbit (94%), Hamster, Pig (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu VIPR1

Target: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Andere Bezeichnung: VIPR1

Hintergrund: Name/Gene ID: VIPR1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR

Synonyms: VIPR1, HVR1, PACAP type II receptor, PACAP-R-2, Pvr2, RDC1, Vip receptor subtype 1, Vpac1 receptor, V1RG, VIP receptor 1, VIPR, VIRG, VPCAP1R, PACAP-R2, VAPC1, VIP and PACAP receptor 1, VPAC1R, Pacap receptor, type ii, Type II PACAP receptor, VIP receptor, type I, VIP-R-1, VIP1 receptor, VPAC1

Gen-ID: 7433