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MRGPRE Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MRGPRE in IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Affe und wurde in 1 Publikation erwähnt.
Produktnummer ABIN1049071

Kurzübersicht für MRGPRE Antikörper (C-Term) (ABIN1049071)

Target

Alle MRGPRE Antikörper anzeigen
MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Reaktivität

  • 8
  • 8
  • 5
  • 1
Human, Affe

Wirt

  • 11
Kaninchen

Klonalität

  • 11
Polyklonal

Konjugat

  • 11
Dieser MRGPRE Antikörper ist unkonjugiert

Applikation

  • 8
  • 5
  • 3
  • 2
  • 1
  • 1
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    C-Term

    Marke

    IHC-plus™

    Spezifität

    Human MRGPRE. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 19 amino acid peptide from C-terminus of human MRGPRE. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (5 - 12 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Zhang, Taylor, Xie, Ford, Johnson, Paulsen, Bates: "Cloning and expression of MRG receptors in macaque, mouse, and human." in: Brain research. Molecular brain research, Vol. 133, Issue 2, pp. 187-97, (2005) (PubMed).

  • Target

    MRGPRE (MAS-Related GPR, Member E (MRGPRE))

    Andere Bezeichnung

    MRGPRE / MRGE

    Hintergrund

    Name/Gene ID: MRGPRE
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: MRGPRE, G protein-coupled receptor 167, G-protein coupled receptor 167, MAS-related GPR, member E, GPR167, MRGE

    Gen-ID

    116534
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