MRGPRE Antikörper (3rd Intracellular Loop)

Produktnummer ABIN7886568

Der Kaninchen Polyklonal anti-MRGPRE Antikörper (ABIN7886568) detektiert spezifisch MRGPRE in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für MRGPRE Antikörper (3rd Intracellular Loop) (ABIN7886568)

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRGPRE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MRGPRE Antikörper

Bindungsspezifität: 3rd Intracellular Loop, AA 197-209

Verwendungszweck: A Rabbit Polyclonal Antibody to MRGPRE Receptor

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 10,13 amino acid residues identical

Produktmerkmale: Anti-MRGPRE Antibody (ABIN7043319, ABIN7044604 and ABIN7044605) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235909)

Blocking Peptide: (ABIN7235909)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu MRGPRE

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Andere Bezeichnung: MRGPRE

Hintergrund:

Synonyms: Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167

Description: The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX1,2.MRGPRE consist of only one receptor per species and is conserved in rodents and primates2. MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an "ionic-lock" known to inhibit activation and activity in some class A GPCRs1,2.Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons1,2.Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury2.

Gen-ID: 404660

NCBI Accession: NM_001039165

UniProt: Q7TN40