LOXL2 Antikörper (Internal Region)

Produktnummer ABIN1049011

Produktdetails anti-LOXL2 Antikörper

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Meerschweinchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LOXL2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human LOXL2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Guinea pig (100%) Bat (95%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from internal region of human LOXL2. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Guinea pig (100%), Bat (95%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (15 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu LOXL2

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Andere Bezeichnung: LOXL2 (LOXL2 Produkte)

Synonyme: LOR2 antikoerper, WS9-14 antikoerper, CG4402 antikoerper, Dmel\\CG4402 antikoerper, Dmloxl-2 antikoerper, dmlox-2 antikoerper, 1110004B06Rik antikoerper, 4930526G11Rik antikoerper, 9430067E15Rik antikoerper, GB13360 antikoerper, lox2-like antikoerper, LOXL2 antikoerper, lor2 antikoerper, loxl-2 antikoerper, ws9-14 antikoerper, im:7136137 antikoerper, si:dkeyp-32b1.1 antikoerper, wu:fk12g04 antikoerper, zgc:158414 antikoerper, lysyl oxidase like 2 antikoerper, lysyl oxidase-like 2 antikoerper, lysyl oxidase homolog 4 antikoerper, lysyl oxidase like 2 L homeolog antikoerper, lysyl oxidase-like 2b antikoerper, LOXL2 antikoerper, lox2 antikoerper, Loxl2 antikoerper, LOC408544 antikoerper, loxl2.L antikoerper, loxl2 antikoerper, loxl2b antikoerper

Hintergrund: Name/Gene ID: LOXL2

Synonyms: LOXL2, Lysyl oxidase-like protein 2, Lysyl oxidase related 2, Lysyl oxidase-like 2, WS9-14, LOR2, Lysyl oxidase homolog 2

Gen-ID: 4017

Pathways: Chromatin Binding