FZD3 Antikörper (N-Term)

Produktnummer ABIN1048620

Dieses Anti-FZD3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FZD3 in IHC und IHC (p). Geeignet für Human, Maus, Ratte, Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Affe, Schwein, Kaninchen und Huhn.

Kurzübersicht für FZD3 Antikörper (N-Term) (ABIN1048620)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reaktivität: Human, Maus, Ratte, Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Affe, Schwein, Kaninchen, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FZD3 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human FZD3 / Frizzled 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD6 (39 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Xenopus (89%) Platypus, Zebrafish (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FZD3 / Frizzled 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Xenopus (89%), Platypus, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Andere Bezeichnung: FZD3 / Frizzled 3

Hintergrund: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

Gen-ID: 7976

Pathways: WNT Signalweg, Tube Formation