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Adenosine A3 Receptor Antikörper (Cytoplasmic Domain)

ADORA3 Reaktivität: Human, Schwein, Hund, Pferd, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048307
  • Target Alle Adenosine A3 Receptor (ADORA3) Antikörper anzeigen
    Adenosine A3 Receptor (ADORA3)
    Bindungsspezifität
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 31
    • 15
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Schwein, Hund, Pferd, Affe
    Wirt
    • 32
    • 1
    • 1
    Kaninchen
    Klonalität
    • 33
    • 1
    Polyklonal
    Konjugat
    • 26
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Adenosine A3 Receptor Antikörper ist unkonjugiert
    Applikation
    • 21
    • 13
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Dog, Horse, Pig, Opossum (100%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human ADORA3. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Dog, Horse, Pig, Opossum (100%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ADORA3 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (20 - 30 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Adenosine A3 Receptor (ADORA3)
    Andere Bezeichnung
    ADORA3 / Adenosine A3 Receptor (ADORA3 Produkte)
    Synonyme
    A3AR antikoerper, AD026 antikoerper, bA552M11.5 antikoerper, ADORA3 antikoerper, 1700001D09Rik antikoerper, 4930578J19Rik antikoerper, A3R antikoerper, AA3R antikoerper, ARA3 antikoerper, Gpcr2 antikoerper, adenosine A3 receptor antikoerper, adenosine receptor A3 antikoerper, ADORA3 antikoerper, Adora3 antikoerper, LOC100603318 antikoerper
    Hintergrund
    Name/Gene ID: ADORA3
    Subfamily: Adenosine
    Family: GPCR

    Synonyms: ADORA3, A3AR, AD026, Adenosine A3 receptor, BA552M11.5, A3 adenosine receptor, Adenosine receptor A3, RP11-552M11.7
    Gen-ID
    140
    Pathways
    Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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