Serotonin Receptor 1E Antikörper (Cytoplasmic Domain)
Kurzübersicht für Serotonin Receptor 1E Antikörper (Cytoplasmic Domain) (ABIN1048261)
Target
Alle Serotonin Receptor 1E (HTR1E) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
- 
    - 
                                            Bindungsspezifität
- Cytoplasmic Domain
- 
                                            Marke
- IHC-plus™
- 
                                            Spezifität
- Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HTR1F (65 % ).
- 
                                            Homologie
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Horse, Guinea pig, Turkey, Sparrow, Chicken, Lizard (100%) Bat, Opossum (94%) Stickleback, Zebrafish (88%).
- 
                                            Aufreinigung
- Immunoaffinity purified
- 
                                            Immunogen
- 
                        Synthetic 17 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Horse, Guinea pig, Turkey, Sparrow, Chicken, Lizard (100%), Bat, Opossum (94%), Stickleback, Zebrafish (88%).
 Type of Immunogen: Synthetic peptide
 
- 
                                            
- 
    
- 
    - 
                                            Applikationshinweise
- 
                        Approved: IHC, IHC-P (10 μg/mL)
 Usage: Antigen retrieval requires Proteinase K treatment.
- 
                                            Kommentare
- 
                        Target Species of Antibody: Human 
- 
                                            Testdurchführung
- 
                        The IHC-pro Immunohistochemistry Protocol 
 Tissue Preparation
 Formalin fixation and embedding in paraffin wax
 Tissue Sectioning
 Make 4-µm sections and place on pre-cleaned and charged microscope slides.
 Heat in a tissue-drying oven for 45 minutes at 60°C
 Deparaffinization
 Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
 Rehydration
 Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
 Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
 Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
 Rinse slides in gentle running distilled water – 5 minutes at room temperature.
 Antigen retrieval
 Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
 Remove from heat and let stand at room temperature in buffer - 20 minutes
 Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
 Immunostaining
 Do not allow tissues to dry at any time during the staining procedure.
 Apply a universal protein block – 20 minutes at room temperature.
 Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
 Rinse slides in 1X TBST - 1 minute at room temperature.
 Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
 Rinse slides 1X TBST – 1 minute at room temperature.
 Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
 Rinse slides in 1X TBST - 1 minute at room temperature.
 Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
 Wash slides in distilled water – 1 minute at room temperature.
 Dehydrate
 This method should only be used if the chromogen substrate is alcohol insoluble.
 Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
 Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
 Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
 Wash slides in 3 changes of xylene – 1 minute each at room temperature.
 Apply coverslip
- 
                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
- 
                                            
- 
    - 
                                            Format
- Liquid
- 
                                            Konzentration
- Lot specific
- 
                                            Buffer
- PBS, less than 0.1 % sodium azide.
- 
                                            Konservierungsmittel
- Sodium azide
- 
                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 
                                            Lagerung
- 4 °C,-20 °C
- 
                                            Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 
                                            Haltbarkeit
- 12 months
 
- 
                                            
- 
    - Serotonin Receptor 1E (HTR1E)
- 
                                            Andere Bezeichnung
- HTR1E / 5-HT1E Receptor
- 
                                            Hintergrund
- 
                        Name/Gene ID: HTR1E
 Subfamily: Serotonin
 Family: GPCR
 Synonyms: HTR1E, 5-HT1E, 5-HT1e receptor, 5-HT-1E, 5HT1E Receptor, HT1e receptor, S31, Serotonin 5-HT-1e receptor, Serotonin 1e receptor, Serotonin receptor 1E
- 
                                            Gen-ID
- 3354
- 
                                            Pathways
- JAK-STAT Signalweg, cAMP Metabolic Process
 Target
- 
                    
 
                                     
                                     
                                    