Serotonin Receptor 1E Antikörper (Cytoplasmic Domain)
Kurzübersicht für Serotonin Receptor 1E Antikörper (Cytoplasmic Domain) (ABIN1048260)
Target
Alle Serotonin Receptor 1E (HTR1E) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- Cytoplasmic Domain
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Marke
- IHC-plus™
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Spezifität
- Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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Homologie
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Guinea pig (100%) Panda, Opossum, Turkey, Sparrow, Chicken (94%) Lizard (88%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Guinea pig (100%), Panda, Opossum, Turkey, Sparrow, Chicken (94%), Lizard (88%).
Type of Immunogen: Synthetic peptide
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Applikationshinweise
- Approved: IHC, IHC-P (16 - 24 μg/mL)
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Kommentare
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Target Species of Antibody: Human
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Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, less than 0.1 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- Serotonin Receptor 1E (HTR1E)
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Andere Bezeichnung
- HTR1E / 5-HT1E Receptor
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Hintergrund
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Name/Gene ID: HTR1E
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR1E, 5-HT1E, 5-HT1e receptor, 5-HT-1E, 5HT1E Receptor, HT1e receptor, S31, Serotonin 5-HT-1e receptor, Serotonin 1e receptor, Serotonin receptor 1E -
Gen-ID
- 3354
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Pathways
- JAK-STAT Signalweg, cAMP Metabolic Process
Target
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