TUBA1B Antikörper (AA 425-451) (Biotin)

Produktnummer ABIN1043919

Hersteller Produkt- Nr.: 600-406-880

Dieser Anti-TUBA1B Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von TUBA1B in WB, ELISA, IHC und FM. Geeignet für Human, Maus, Ratte, Huhn, Rind (Kuh), Ziege und Schaf. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für TUBA1B Antikörper (AA 425-451) (Biotin) (ABIN1043919)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Ziege, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TUBA1B Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Produktdetails anti-TUBA1B Antikörper (Biotin)

Bindungsspezifität: AA 425-451

Hersteller Produkt- Nr.: 600-406-880

Hersteller: Rockland

Verwendungszweck: Alpha-Tubulin Antibody Biotin Conjugated

Kreuzreaktivität (Details): Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.

Produktmerkmale: Synonyms: rabbit anti-alpha-Tubulin Antibody biotin conjugation, biotin conjugated rabbit anti-α-tubulin antibody, biotin conjugated rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody biotin conjugation, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Aufreinigung: The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000-1:100,000

IF Microscopy Dilution: 1:500 - 1:2,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.61 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TUBA1B Antikörper (Biotin) (ABIN1043919)

Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).

Details zu TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Andere Bezeichnung: TUBA1B

Hintergrund: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase