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GDF15 Antikörper (C-Term) (HRP)

Der Rabbit Polyclonal Anti-GDF15-Antikörper wurde für WB, ELISA, IHC validiert. Er ist geeignet, GDF15 in Proben von Human, Mouse zu detektieren. Es ist 1 Publikation verfügbar.
Rockland
Produktnummer ABIN1043915
Hersteller Produkt- Nr.: 600-403-b07

Kurzübersicht für GDF15 Antikörper (C-Term) (HRP) (ABIN1043915)

Target

Alle GDF15 Antikörper anzeigen
GDF15 (Growth Differentiation Factor 15 (GDF15))

Reaktivität

  • 116
  • 40
  • 26
  • 1
  • 1
Human, Maus

Wirt

  • 106
  • 26
  • 3
  • 2
  • 1
Kaninchen

Klonalität

  • 103
  • 32
  • 2
Polyklonal

Konjugat

  • 83
  • 18
  • 10
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GDF15 Antikörper ist konjugiert mit HRP

Applikation

  • 112
  • 67
  • 61
  • 28
  • 23
  • 15
  • 13
  • 13
  • 7
  • 6
  • 5
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 13
    • 11
    • 9
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Hersteller Produkt- Nr.

    600-403-b07

    Hersteller

    Rockland

    Verwendungszweck

    GDF15 Antibody Peroxidase Conjugated

    Kreuzreaktivität (Details)

    This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.

    Produktmerkmale

    Synonyms: rabbit anti-NAG1 antibody peroxidase conjugation, HRP conjugated rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor

    Aufreinigung

    Anti-NAG-1 (C-terminal specific) was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    Immunogen

    Immunogen: Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:500-1:2,500

    Application Note: Anti-NAG1/GDF15 (C-terminal specific) antibody has been tested by ELISA and is suitable for IHC and western blotting of human and mouse NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000-1:5,000

    ELISA Dilution: 1:10,000-1:50,000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    1.07 mg/ml

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Konservierungsmittel

    Gentamicin sulfate

    Vorsichtsmaßnahmen

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).

  • Target

    GDF15 (Growth Differentiation Factor 15 (GDF15))

    Andere Bezeichnung

    GDF15

    Hintergrund

    Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

    Gen-ID

    9518

    UniProt

    Q99988

    Pathways

    SARS-CoV-2 Protein Interaktom
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