IDO1 Antikörper

Produktnummer ABIN1043819

Hersteller Produkt- Nr.: 210-301-e58

Produktdetails anti-IDO1 Antikörper

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IDO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Hersteller Produkt- Nr.: 210-301-e58

Hersteller: Rockland

Verwendungszweck: IDO1 Antibody

Kreuzreaktivität (Details): IDO1 antibody is specific for mouse IDO1 protein.

Produktmerkmale: Synonyms: mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido 1, Ido-1, IDO1 antibody, mouse anti-IDO-1 antibody

Aufreinigung: Anti-IDO1 antibody was purified from ascites fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Klon: 2E2-6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 0.5-1x10^6 cells

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

Immunoprecipitation Dilution: 10-100 μL

ELISA Dilution: 1:5000-1:50000

IF Microscopy Dilution: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-IDO1 Antikörper (ABIN1043819)

Mellor, Munn: "IDO expression by dendritic cells: tolerance and tryptophan catabolism." in: Nature reviews. Immunology, Vol. 4, Issue 10, pp. 762-74, (2004) (PubMed).

Details zu IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Andere Bezeichnung: Ido1 (IDO1 Produkte)

Hintergrund: Background: Anti-IDO-1 antibody recognizes indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Gen-ID: 15930

NCBI Accession: NP_032350

UniProt: P28776

Pathways: Activated T Cell Proliferation