PDCD4 Antikörper (pSer457) (Biotin)

Produktnummer ABIN1043755

Hersteller Produkt- Nr.: 200-306-964

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PDCD4 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Xenopus laevis und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für PDCD4 Antikörper (pSer457) (Biotin) (ABIN1043755)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDCD4 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 9G6

Produktdetails anti-PDCD4 Antikörper (Biotin)

Bindungsspezifität: pSer457

Hersteller Produkt- Nr.: 200-306-964

Hersteller: Rockland

Verwendungszweck: Pdcd4 phospho S457 Antibody Biotin Conjugated

Kreuzreaktivität (Details): This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.

Produktmerkmale: Synonyms: mouse anti-Pdcd4 pS457 Antibody biotin conjugation, biotin conjugated mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Aufreinigung: Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography.

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-PDCD4 Antikörper (Biotin) (ABIN1043755)

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4

Hintergrund: Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Gen-ID: 27250, 21735596

UniProt: Q53EL6