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HIF3A Antikörper (AA 575-600)

Der Kaninchen Polyklonal Anti-HIF3A-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, HIF3A in Proben von Maus zu detektieren. Es ist 1 Publikation verfügbar.
Rockland
Produktnummer ABIN100475
Hersteller Produkt- Nr.: 600-401-435

Kurzübersicht für HIF3A Antikörper (AA 575-600) (ABIN100475)

Target

Alle HIF3A Antikörper anzeigen
HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Reaktivität

  • 25
  • 9
  • 7
  • 1
Maus

Wirt

  • 21
  • 4
  • 1
Kaninchen

Klonalität

  • 25
  • 1
Polyklonal

Konjugat

  • 22
  • 2
  • 1
  • 1
Dieser HIF3A Antikörper ist unkonjugiert

Applikation

  • 20
  • 12
  • 11
  • 5
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA
  • Bindungsspezifität

    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 575-600

    Hersteller Produkt- Nr.

    600-401-435

    Hersteller

    Rockland

    Verwendungszweck

    Hif3 alpha Antibody

    Kreuzreaktivität (Details)

    This affinity-purified antibody is directed against mouse Hif3alpha protein.

    Produktmerkmale

    Synonyms: rabbit anti-Hif3a antibody, Hif3 alpha antibody, Hif3α antibody, Hypoxia Inducible Factor 3-alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Inhibitory PAS domain protein antibody, IPAS antibody, HIF-3-alpha, Basic-helix-loop-helix-PAS protein MOP7, HIF3-alpha-1, Neonatal and embryonic PAS protein

    Aufreinigung

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.

    Western Blot Dilution: 1:1,000 - 1:8,000

    ELISA Dilution: 1:14,000 - 1:80,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.2 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Takeda, Ho, Takeda, Duan, Nagy, Fong: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, (2006) (PubMed).

  • Target

    HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

    Andere Bezeichnung

    Hif3a

    Hintergrund

    Background: One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF binds to the hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-a may be a marker for tumor growth and apoptosis. Hif3 may participate in disorders with the cornea, lung, and heart. Anti-Hif3 alpha Antibody is useful for researchers interested in transcription factors, DNA binding, and Epilepsy research.

    Gen-ID

    53417, 170295859

    UniProt

    Q0VBL6

    Pathways

    Warburg Effekt
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