serine Peptidase Inhibitor, Kunitz Type, 2 Proteine (SPINT2)

Human serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2) Interaktionspartner

1. The expression of SPINT2 gene is regulated by its methylation status, and the methylation status of SPINT2 is altered by HPV infection. The aberrant methylation status of SPINT2 gene may play an important role in the development of cervical cancer.

2. aberrant methylation of the SPINT2 gene is frequently observed in high-grade gliomas and might confer MET signaling in the glioma cells.

3. finding of only one heterozygous SPINT2 mutation in 19 patients with isolated CA/GA was not statistically significant

4. In this work, KLK14 (zeige KLK14 Proteine) binding to either hepatocyte growth factor activator inhibitor type-1 (HAI-1 (zeige SPINT1 Proteine)) or type-2 (HAI-2) was essayed using homology modeling, molecular dynamic simulations and free-energy calculations through MM/PBSA and MM/GBSA. KLK14 (zeige KLK14 Proteine) was successfully modeled.

5. limited role for HAI-2 in the inhibition of matriptase (zeige ST14 Proteine) and prostasin (zeige PRSS8 Proteine) is the result of its primarily intracellular localization in basal and spinous layer keratinocytes, which probably prevents the Kunitz inhibitor from interacting with active prostasin (zeige PRSS8 Proteine) or matriptase (zeige ST14 Proteine)

6. that the HAI-2 Kunitz domain 1 mutants influence the flux of matriptase (zeige ST14 Proteine) to the plasma membrane by affecting the oligomerization, maturation and/or folding of matriptase (zeige ST14 Proteine)

7. Our data indicate that hypoxic inhibition of JMJD3 activity reduces demethylation of H3K27me3, nucleosome removal, and hence induction of the STAT6 target gene CCL18, while induction of other STAT6-inducible genes such as SPINT2 remained unaffected by JMJD3.

8. Concomitant presence of TMPRSS13 (zeige TMPRSS13 Proteine) with HAI-2 mediates phosphorylation of residues in the intracellular domain of the protease, and it coincides with efficient transport of the protease to the cell surface and its subsequent shedding.

9. This study presented a molecular characterization of congenital tufting enteropathy Italian patients, and identified one mutation in the SPINT2 gene

10. study the methylation status of the promoter region of Serine peptidase inhibitor/hepatocyte growth factor activator inhibitor type 2 (SPINT2/HAI-2)

Mouse (Murine) serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2) Interaktionspartner

1. Intestines of HAI-2 deficient mice showed altered expression of epithelial junctional proteins, including reduced levels of EpCAM (zeige EPCAM Proteine), E-cadherin (zeige CDH1 Proteine), occludin (zeige OCLN Proteine), claudin-1 (zeige CLDN1 Proteine) and -7, as well as an increased level of claudin-4 (zeige CLDN4 Proteine), indicating that the loss of HAI-2 compromises intestinal epithelial barrier function.

2. HAI-1 (zeige SPINT1 Proteine) regulates the activity of activated matriptase (zeige ST14 Proteine), whereas HAI-2 has an essential role in regulating prostasin (zeige PRSS8 Proteine)-dependent matriptase (zeige ST14 Proteine) zymogen activation.

3. mutations in Prss8 (zeige PRSS8 Proteine) restored placentation and normal development of HAI-1 (zeige SPINT1 Proteine)-deficient embryos and prevented early embryonic lethality, mid-gestation lethality due to placental labyrinth failure, and neural tube defects in HAI-2-deficient embryos.

4. HAI-1 (zeige SPINT1 Proteine) and -2 are overexpressed in the liver in cholangiopathies with ductular reactions and are possibly involved in liver fibrosis and hepatic differentiation.

5. Unlike HAI-1 (zeige SPINT1 Proteine) and matriptase (zeige ST14 Proteine), HAI-2 expression is detected in non-epithelial cells of brain and lymph nodes, suggesting that HAI-2 may also be involved in inhibition of serine proteases other than matriptase (zeige ST14 Proteine)

6. Inhibition of the transmembrane serine protease (zeige F2 Proteine) matriptase (zeige ST14 Proteine) (encoded by St14 (zeige ST14 Proteine)) is an essential function of HAI2 during tissue morphogenesis.