Sonic Hedgehog Protein (SHH) (AA 25-198) (His tag)

Produktnummer ABIN666981

Dieses Recombinant Sonic Hedgehog-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Sonic Hedgehog Protein (SHH) (AA 25-198) (His tag) (ABIN666981)

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 25-198

Aufreinigungstag / Konjugat: Dieses Sonic Hedgehog Protein ist gelabelt mit His tag.

Sequenz: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH

Produktmerkmale: SHH, 25-198 aa, Mouse, His-tagged, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Sonic Hedgehog/Shh

Hintergrund: SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 20.8 kDa (183aa) confirmed by MALDI-TOF

NCBI Accession: NP_033196

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development