HMGB1 Protein (AA 1-215) (His tag)
Kurzübersicht für HMGB1 Protein (AA 1-215) (His tag) (ABIN666708)
Target
Alle HMGB1 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-215
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Aufreinigungstag / Konjugat
- Dieses HMGB1 Protein ist gelabelt mit His tag.
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Sequenz
- MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDE
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Produktmerkmale
- HMGB1, 1-215 aa, Human, His tagged, Recombinant, Baculovirus
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Aufreinigung
- > 90% by SDS-PAGE
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Endotoxin-Niveau
- < 1 EU per 1ug of protein (determined by LAL method)
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 Spezies: Human, Maus, Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (C106S), (C23S), (C45S), (Non-oxidizable) (Active) proteinHMGB1 Spezies: Human, Maus, Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 Spezies: Human Wirt: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM EDTA, 0.5 mM DTT, 10 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Andere Bezeichnung
- HMG-1/HMGB1
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Hintergrund
- High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in insect cells and purified by conventional chromatography techniques.
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Molekulargewicht
- 25 kDa (223aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002119
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Pathways
- p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target
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