M-CSF protein (HIS)

Name: M-CSF

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN666654

Produktbeschreibung

Produktmerkmale: MCSF, 33-190 aa, Human, His-tagged, E.coli

Format: Liquid

Beschreibung: Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. . Synonyms: CSF1 , Macrophage colony stimulating factor Colony stimulating factor 1, CSF 1, Lanimostim, M CSF, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930. NCBI no.: NP_757351

Molekulargewicht: 20.7 kDa (179aa), confirmed by MALDI-TOF.

Anwendungen

Konzentration: 1 mg/ml (determined by Bradford assay)

Reinheit: > 90% by SDS - PAGE

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0), 2mM DTT, 10% glycerol

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

M-CSF protein (HIS)