HMGB1 Protein (His tag)

Produktnummer ABIN623045

Recombinant HMGB1-Protein exprimiert in not_set.

Kurzübersicht für HMGB1 Protein (His tag) (ABIN623045)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Spezies: Human

Wirt: Bitte anfragen

Reinheit: > 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses HMGB1 Protein ist gelabelt mit His tag.

Sequenz: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDELEHHHH HH

Produktmerkmale: Recombinant Human High-Mobility Group Box 1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4.It is recommended to reconstitute the lyophilized HMGB1 in sterile 18M -cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Lagerung: -20 °C

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-Mobility Group Box 1

Hintergrund: HMG1 Human Recombinant fused with 6X His tag produced in E. coli is a single, non-glycosylated, polypeptide chain containing 223 AA and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques. Introduction: High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 AA (AA) single chain polypeptide containing three domains: two N-terminal globular, 70 AA positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 AA C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. Synonyms: HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome