Activin A (ACVA) ELISA Kit

Antigen: Activin A (ACVA)

Reaktivität: Ratte (Rattus)

Applikation: ELISA

Produktnummer: ABIN411256

Produktbeschreibung

Produktmerkmale: For quantitative detection of rat Activin A in sera, body fluids, tissue lysates or cell culture supernates.

Weitere Bezeichnung: Activin A

Beschreibung: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Spezifität: No detectable cross-reactivity with any other cytokine.

Sensitivität: Sensitivity: < 12pg/ml. Range: 62.5pg/ml-4000pg/ml

Anwendungen

Prinzip: This rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat Activin A specific-specific monoclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies (clone No. 69403) were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Bestandteile: Lyophilized recombinant rat Activin A standard: 10ng/tube

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Lagerung: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Activin A (ACVA) ELISA Kit