RPS6KB1 Antikörper (AA 2-121)

Produktnummer ABIN968441

Produktdetails anti-RPS6KB1 Antikörper

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Bindungsspezifität: AA 2-121

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RPS6KB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p70 [S6K] aa. 2-121

Klon: 16-p70[s6k]

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968548, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RPS6KB1 Antikörper (ABIN968441)

Brennan, Babbage, Thomas, Cantrell: "p70(s6k) integrates phosphatidylinositol 3-kinase and rapamycin-regulated signals for E2F regulation in T lymphocytes." in: Molecular and cellular biology, Vol. 19, Issue 7, pp. 4729-38, (1999) (PubMed).

Johanson, Naccache, Crouch: "A p85 subunit-independent p110alpha PI 3-kinase colocalizes with p70 S6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in swiss 3T3 cells." in: Experimental cell research, Vol. 248, Issue 1, pp. 223-33, (1999) (PubMed).

Romanelli, Martin, Toker, Blenis: "p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex." in: Molecular and cellular biology, Vol. 19, Issue 4, pp. 2921-8, (1999) (PubMed).

Ming, Burgering, Wennström, Claesson-Welsh, Heldin, Bos, Kozma, Thomas: "Activation of p70/p85 S6 kinase by a pathway independent of p21ras." in: Nature, Vol. 371, Issue 6496, pp. 426-9, (1994) (PubMed).

Details zu RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Andere Bezeichnung: p70 s6k (RPS6KB1 Produkte)

Hintergrund: Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6. p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases. p70 [S6K] and p85 [S6K] are two isoforms of the same kinase. Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence. These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation. Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389. p70 [S6K] associates with PDK-1 and PKCzeta to form a possible PI3-K signaling complex. The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation. Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses. p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.
Synonyms: p70 S6 Kinase

Molekulargewicht: 60-70 kDa

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effekt