RAP1 Antikörper (AA 1-184)

Produktnummer ABIN967814

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-RAP1 Antikörper wird verwendet zum Nachweis von RAP1 in Proben von Human, Maus, Ratte, Huhn und Frosch. Er wurde validiert für WB, IP, IF und IHC (f).

Kurzübersicht für RAP1 Antikörper (AA 1-184) (ABIN967814)

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Reaktivität: Human, Maus, Ratte, Huhn, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 3-Rap1

Produktdetails anti-RAP1 Antikörper

Bindungsspezifität: AA 1-184

Kreuzreaktivität: Huhn, Frosch, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rap1 aa. 1-184

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RAP1 Antikörper (ABIN967814)

Larson, Chen, Kahn, Taylor, Fabre, Mortensen, Conley, Parise: "Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets." in: Blood, Vol. 101, Issue 4, pp. 1409-15, (2003) (PubMed).

Wu, Lai, Mobley: "Nerve growth factor activates persistent Rap1 signaling in endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 15, pp. 5406-16, (2001) (PubMed).

Xing, Ge, Zeltser, Maskevitch, Mayer, Alexandropoulos: "c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase." in: Molecular and cellular biology, Vol. 20, Issue 19, pp. 7363-77, (2000) (PubMed).

Okada, Pessin: "Insulin and epidermal growth factor stimulate a conformational change in Rap1 and dissociation of the CrkII-C3G complex." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28179-82, (1997) (PubMed).

Yamamoto, Kaibuchi, Mizuno, Hiroyoshi, Shirataki, Takai: "Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins." in: The Journal of biological chemistry, Vol. 265, Issue 27, pp. 16626-34, (1990) (PubMed).

Details zu RAP1

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Andere Bezeichnung: Rap1

Hintergrund: Rap1 is a member of the large Ras superfamily of low molecular weight GTP/GDP binding proteins. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap1A and Rap1B are highly homologous proteins, differing in only 9 of their 184 amino acids. Overexpression of Rap1A (also known as Krev-1) causes reversion of the phenotype of a Ki-Ras-transformed cell line. In vitro, Rap1 can compete efficiently with p21ras for interaction with Ras-GAP. Though they appear to have similar activities, Rap1 and Ras differ in their cellular localization. Ras is found on the inner surface of the plasma membrane while Rap1 is associated with the Golgi. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 21 kDa

Pathways: Zellzyklus, Telomere Maintenance