LAT2 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN94439

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-LAT2 Antikörper wird verwendet zum Nachweis von LAT2 in Proben von Human und Maus. Er wurde validiert für WB, IP, ICC und ICFC.

Kurzübersicht für LAT2 Antikörper (Cytoplasmic Domain) (ABIN94439)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Klon: NAP-07

Produktdetails anti-LAT2 Antikörper

Bindungsspezifität: AA 91-243, Cytoplasmic Domain

Verwendungszweck: Anti-NTAL Purified

Kreuzreaktivität (Details): Mouse, Human, Other not tested

Produktmerkmale: The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1-2 μg/mL.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-LAT2 Antikörper (ABIN94439)

Duque-Afonso, Yalcin, Berg, Abdelkarim, Heidenreich, Lübbert: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, (2011) (PubMed).

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, (2007) (PubMed).

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Details zu LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Andere Bezeichnung: NTAL

Hintergrund: Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2

Gen-ID: 56743

UniProt: Q9JHL0

Pathways: Fc-epsilon Rezeptor Signalübertragung, BCR Signaling