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Antikörper
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anti-Cytokeratin 18 (KRT18) Antikörper
Cytokeratin 18 (KRT18) Antikörper
| Antigen |
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| Synonyme |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Klonalität |
Monoklonal (DC-10) |
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Wirt
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Reaktivität
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Human (187), Maus (70), Ratte (Rattus) (68), Hund (45), Kaninchen (35), Rind (Kuh) (27), Schwein (23), Huhn (20), Pferd (17), Säugetiere (10), Hamster (6), None (4), Schaf (4), Vogel (2), Katze (2), Affe (2)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Immunzytochemie (ICC)
Western Blot (WB) (116), Immunhistochemie (Paraffinschnitte) (IHC (p)) (111), Immunfluoreszenz (IF) (86), Durchflusszytometrie (FACS) (69), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (51), Immunhistochemie (IHC) (34), Immunpräzipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (23), Immunzytochemie (ICC) (13), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN94282 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
180,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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Cytokeratin 18
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Gen-ID
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17q24.2
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Immunogen
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Human breast carcinoma cell line PMC-42.
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Format
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Purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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DC-10
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Beschreibung
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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).
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Spezifität
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The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Dog (Canine).
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Applikationshinweise
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Western Blotting - Recommended dilution: 1-2 µg/ml, overnight in 4 o C Positive control: HeLa human cervix carcinoma cell lineMCF-7 human breast adenocarcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel). Immunohistochemistry (paraffin sections) - Recommended dilution: 1: 10 - 1: 100Positive tissue: epithelial tissue (skin) -. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell lineHeLa human cervix carcinoma cell lineELISA - Positive control: MCF-7 human breast adenocarcinoma cell line. Application note: The antibody DC-10 has been tested as the detection antibody in asandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Signaltransduktion, Zytoskelett, Extrazelluläre Matrix
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Ta: "Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, 2011 (PubMed).
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Alternativen zu Antigen "Cytokeratin 18 (KRT18)", Typ "Antikörper" finden
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Wirte
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Maus (124), Kaninchen (93), Huhn (2), Meerschweinchen (1), Schaf (1)
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Reaktivitäten
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Human (187), Maus (70), Ratte (Rattus) (68), Hund (45), Kaninchen (35), Rind (Kuh) (27), Schwein (23), Huhn (20), Pferd (17), Säugetiere (10), Hamster (6), None (4), Schaf (4), Vogel (2), Katze (2), Affe (2)
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Applikationen
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Western Blot (WB) (116), Immunhistochemie (Paraffinschnitte) (IHC (p)) (111), Immunfluoreszenz (IF) (86), Durchflusszytometrie (FACS) (69), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (51), Immunhistochemie (IHC) (34), Immunpräzipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (23), Immunzytochemie (ICC) (13), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (1)
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Konjugate
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Biotin (17), FITC (14), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitope
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pSer33 (10), C-Term (5), AA 7-173 (1), Center (1), N-Term (1)
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