CD18 Antikörper

Antigen: CD18

Klonalität: Monoklonal (MEM-148)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Funktionale Studien (Func)

Produktnummer: ABIN94010

Produktbeschreibung

Immunogen: Peripheral blood mononuclear cells

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-148

Beschreibung: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Spezifität: The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible inintact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa typeI transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052 This antibody does not react with: Porcine.

Anwendungen

Applikationshinweise: Flow Cytometry: The antibody MEM-148 is excellent marker of activated myeloid cells. Application note: Non-reducing conditions. Functional Application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1conformation in resting leukocytes.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).