PRKAG3 Antikörper (AA 151-250) (Cy5)

Produktnummer ABIN751877

Der Kaninchen Polyklonal anti-PRKAG3 Antikörper wird verwendet zum Nachweis von PRKAG3 in Proben von Human. Er wurde validiert für WB, IF (cc) und IF (p).

Kurzübersicht für PRKAG3 Antikörper (AA 151-250) (Cy5) (ABIN751877)

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAG3 Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-PRKAG3 Antikörper (Cy5)

Bindungsspezifität: AA 151-250

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Cow,Sheep,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AMPK gamma 3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PRKAG3

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Andere Bezeichnung: Ampk gamma 3/Prkag3

Hintergrund:

Synonyms: AMPKG3, 5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3

Background: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gen-ID: 53632

UniProt: Q9UGI9

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Warburg Effekt