DICER1 Antikörper

Produktnummer ABIN577054

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DICER1 in WB, IHC, ELISA, IP und ChIP. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio) und Eidechse. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für DICER1 Antikörper (ABIN577054)

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Eidechse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DICER1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-DICER1 Antikörper

Spezifität: Greater than 95%

Aufreinigung: Affinity purified

Reinheit: > 98 %

Immunogen: Synthetic Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Works on IHC at 1/10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1mg/ml

Buffer: PBS/glycerol

Lagerung: -20 °C

Referenzen für anti-DICER1 Antikörper (ABIN577054)

Wang, McAllister, Bailey, Scott, Hendley, Leach, Ghosh: "Dicer is required for maintenance of adult pancreatic acinar cell identity and plays a role in Kras-driven pancreatic neoplasia." in: PLoS ONE, Vol. 9, Issue 11, pp. e113127, (2014) (PubMed).

Caffrey, Ingoldsby, Wall, Webber, Dinneen, Murillo, Inderhaug, Newell, Gupta, Callagy: "Prognostic significance of deregulated dicer expression in breast cancer." in: PLoS ONE, Vol. 8, Issue 12, pp. e83724, (2014) (PubMed).

Jafarnejad, Sjoestroem, Ardekani, Martinka, Li: "Reduced expression of nuclear Dicer correlates with melanoma progression." in: Pigment cell & melanoma research, Vol. 26, Issue 1, pp. 147-9, (2012) (PubMed).

Details zu DICER1

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Andere Bezeichnung: Dicer

Hintergrund: High affinity rabbit derivative of 13D6. Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides long, usually with a two-base overhang on the 3' end. Dicer contains two RNase III domains and one PAZ domain, the distance between these two regions of the molecule is determined by the length and angle of the connector helix and determines the length of the siRNAs it produces. Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand. The human version of this gene is DICER1. In recent work, CLONEGENE has developed a rabbit antibody (R13D6) against a highly conserved peptide in human Dicer. This antibody reacts with human, mouse, rat, cow, dog and lizard Dicer . The sequence of the peptide is confidential but we are happy to advise you if this antibody will work on Dicer from your species of interest. Working conditions are 1/20,000 for IHC and 1/5000 for western blot making this a very economical purchase. R13D6 was generated against the same synthetic peptide that was used to generate our anti-Dicer 13D6 and 4A6 mouse monoclonals. Indeed R12D6 is superior to 4A6 in IHC on archival paraffin sections (see image of R13D6 on FFPE lung tumor section ). R13D6 is provided as an affinity-purified IgG (1mg/ml) .

Pathways: Regulatorische RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization