DICER1 Antikörper (AA 1-195)

Produktnummer ABIN3043347

Produktdetails anti-DICER1 Antikörper

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Bindungsspezifität: AA 1-195

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DICER1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.
Gene Name: dicer 1, ribonuclease type III
Protein Name: Endoribonuclease Dicer

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Dicer is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-DICER1 Antikörper (ABIN3043347)

Gao, Wang, Chen, Feng, Yang, Feng, Yang, Ye, Zhang: "Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells." in: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 898-907, (2017) (PubMed).

Gao, Wang, Feng, Feng, Zheng, Yang, Xiao, Lu, Ye, Zhang: "A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level." in: Biochemical and biophysical research communications, Vol. 459, Issue 2, pp. 306-312, (2015) (PubMed).

Details zu DICER1

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Andere Bezeichnung: DICER1 (DICER1 Produkte)

Synonyme: dcr1 antikoerper, dicer antikoerper, herna antikoerper, DICER1 antikoerper, dicer1 antikoerper, 1110006F08Rik antikoerper, D12Ertd7e antikoerper, mKIAA0928 antikoerper, DCR1 antikoerper, Dicer antikoerper, HERNA antikoerper, MNG1 antikoerper, DICER antikoerper, fc39d11 antikoerper, fc74b05 antikoerper, wu:fc39d11 antikoerper, wu:fc74b05 antikoerper, dicer 1, ribonuclease III antikoerper, endoribonuclease Dicer antikoerper, dicer 1, ribonuclease III S homeolog antikoerper, dicer 1, ribonuclease type III antikoerper, dicer 1 ribonuclease III antikoerper, DICER1 antikoerper, CpipJ_CPIJ010534 antikoerper, dicer1 antikoerper, dicer1.S antikoerper, Dicer1 antikoerper

Hintergrund: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Synonyms: DCR antibody|DCR1 antibody|Dcr1 homolog (Drosophila) antibody|Dicer 1 antibody|Dicer 1 ribonuclease type III antibody|Dicer antibody|DICER_HUMAN antibody|Dicer1 antibody|Double-strand-specific ribonuclease antibody|Endoribonuclease Dicer antibody|Helicase moi antibody|Helicase with RNAse motif antibody|HERNA antibody|KIAA0928 antibody|MNG1 antibody|RMSE2 antibody

Gen-ID: 23405

Pathways: Regulatorische RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization