GFP Antikörper
| Antigen |
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| Klonalität |
Monoklonal (GF28R) |
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Wirt
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Applikation
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 Alternativen Dot Blot (Dot), ELISA, Immunpräzipitation (IP), Immunzytochemie (ICC), Western Blot (WB)
Western Blot (WB) (209), Immunfluoreszenz (IF) (91), ELISA (87), Immunpräzipitation (IP) (87), Durchflusszytometrie (FACS) (64), Enzyme Immunoassay (EIA) (53), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (50), Immunhistochemie (IHC) (26), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Immunzytochemie (ICC) (11), Dot Blot (Dot) (7), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Intrazelluläre Durchflusszytometrie (ICFC) (4), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (2), Immunelektronenmikroskopie (EM) (1)
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| Produktnummer |
ABIN559689 |
| Menge |
100ug (1mg/mL) |
| Preis |
230,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Immunogen
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GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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GF28R
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Spezifität
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Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
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Sensitivität
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WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
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Applikationshinweise
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WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
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Konzentration
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1mg/mL
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Reinigung
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Protein A affinity chromatography from mouse ascites fluid
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Buffer
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10mM PBS, pH 7.2, 0.05% NaN3
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Lagerung
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Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "GFP", Typ "Antikörper" finden
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Wirte
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Kaninchen (139), Maus (83), Ziege (46), Huhn (16), Ratte (3), Schaf (3)
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Reaktivitäten
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Aequorea victoria (81), Human (28), Maus (15), Fusion Protein (Tag) (14), Ratte (Rattus) (13), Alle Spezies (5), Rind (Kuh) (5), Hund (5), Kaninchen (4), Huhn (3), Qualle (1)
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Applikationen
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Western Blot (WB) (209), Immunfluoreszenz (IF) (91), ELISA (87), Immunpräzipitation (IP) (87), Durchflusszytometrie (FACS) (64), Enzyme Immunoassay (EIA) (53), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (50), Immunhistochemie (IHC) (26), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Immunzytochemie (ICC) (11), Dot Blot (Dot) (7), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Intrazelluläre Durchflusszytometrie (ICFC) (4), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (2), Immunelektronenmikroskopie (EM) (1)
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Konjugate
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HRP (21), Biotin (20), FITC (13), Alkaline Phosphatase (AP) (6), Alexa Fluor 647 (5), Texas Red (TR) (4), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3), Agarose Beads (2), Rhodamine (1), TRITC (1)
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Epitope
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N-Term (4)
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