DYKDDDDK (Flag) Tag Antikörper

Antigen: DYKDDDDK (Flag) Tag

Klonalität: Monoklonal (FG4R)

Wirt: Maus

Applikation: Dot Blot (Dot), ELISA, Immunpräzipitation (IP), Immunzytochemie (ICC), Western Blot (WB)

Produktnummer: ABIN559688

Produktbeschreibung

Immunogen: DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: FG4R

Spezifität: Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Sensitivität: WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins

Anwendungen

Applikationshinweise: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Konzentration: 1mg/mL

Reinigung: Protein A affinity chromatography from mouse ascites fluid

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Lagerung: Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector, untransfected (1) and transfected (2)