CTNNB1 Antikörper (pSer37)

Produktnummer ABIN498725

Der Kaninchen Polyklonal anti-CTNNB1 Antikörper wird verwendet zum Nachweis von CTNNB1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für CTNNB1 Antikörper (pSer37) (ABIN498725)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: pSer37

Spezifität: This antibody detects endogenous levels of Catenin-beta protein only when phosphorylated at Ser37.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: Catenin beta-1

Hintergrund: The catenins, alpha, beta and gamma, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser- Pro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.Synonyms: Beta-catenin, CTNNB, CTNNB1

Molekulargewicht: approx. 92 kDa

Gen-ID: 1499

NCBI Accession: NP_001091679

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2