beta-Catenin (CTNNB1) Antikörper

Antigen: beta-Catenin (CTNNB1)

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Klonalität: Monoklonal (EM-22)

Wirt: Maus

Reaktivität: Human, Maus, Hamster

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN457419

Produktbeschreibung

Weitere Bezeichnung: beta-Catenin

Immunogen: Recombinant human beta-catenin

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: EM-22

Beschreibung: Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation oftranscription. Calcium-dependent intercellular adhesion transmembrane glycoproteinE-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta andgamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton.Functional cadherin-catenin system is important for invasiveness of tumour cells.Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. Whenactivity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway),hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocatesto the nucleus and activates transcription of genes including those that are involved in cellcycle control. As a result, cell division and neoplastic transformation are promoted.

Spezifität: The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctionalprotein involved both in cell adhesion and in activation of transcription.

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Anwendungen

Applikationshinweise: Western Blotting - Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1human colon adenocarcinoma cell lineKW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line. Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar