RRM2B Antikörper (N-Term)

Produktnummer ABIN401481

Der Kaninchen Polyklonal anti-RRM2B Antikörper (ABIN401481) detektiert spezifisch RRM2B in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für RRM2B Antikörper (N-Term) (ABIN401481)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RRM2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RRM2B Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody is directed against RRM2B.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Orangutan, Macaque, Porcine, Drosophila.
Species reactivity (tested):Human.

Aufreinigung: Affinity Purified

Immunogen: Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/3,000. Western Blot: 1,0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.82 mg/mL (by UV absorbance at 280nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Andere Bezeichnung: RRM2B / P53R2

Hintergrund: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-ironcarboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein

Gen-ID: 50484

NCBI Accession: NP_001165948

UniProt: Q7LG56

Pathways: p53 Signalweg, Negative Regulation of intrinsic apoptotic Signaling