SMAD3 Antikörper (C-Term)

Produktnummer ABIN401333

Der Kaninchen Polyklonal anti-SMAD3 Antikörper wird verwendet zum Nachweis von SMAD3 in Proben von Human, Maus, Ratte, Schwein, Huhn, Zebrafisch (Danio rerio), Rind (Kuh) und Xenopus laevis. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für SMAD3 Antikörper (C-Term) (ABIN401333)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human, Maus, Ratte, Schwein, Huhn, Zebrafisch (Danio rerio), Rind (Kuh), Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: AA 417-425, C-Term

Spezifität: This antibody is directed against Smad3 protein. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Human Smad3.

Kreuzreaktivität (Details): Species reactivity (expected):Xenopus laevis, Xenopus tropicalis, Zebrafish, Rat, Mouse, Porcine, Bovine, Chicken.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: SMAD3

Hintergrund: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (RSmads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-β pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3

Gen-ID: 4088

NCBI Accession: NP_001138574

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie