GFP Antikörper

Antigen: GFP

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN398857

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.Antibodies-Online Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

Spezifität: Antibodies-Online Rabbit Anti-GFP Polyclonal Antibody reacts with either N-terminal or C-terminal GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlImmunoprecipitation (IP): 2-10 ug/mg of lysateOther Applications: user-optimized

Konzentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-GFP Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of GFP fusion protein using 1 ug/ml Rabbit Anti-GFP Polyclonal Antibody (Antibodies-Online, ABIN398857)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.Predicted Size: 27 KDObserved Size: 27 KD

Western blot analysis of GFPuv fusion protein using 1 ug/ml Rabbit Anti-GFP Polyclonal Antibody (Antibodies-Online, ABIN398857)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.Predicted Size: 27 KDObserved Size: 27 KD

Western blot analysis of YFP fusion protein using 1 ug/ml Rabbit Anti-GFP Polyclonal Antibody (Antibodies-Online, ABIN398857)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.Predicted Size: 40 KDObserved Size: 40 KD