Casein Kinase 1, epsilon (CSNK1E) (C-Term) Antikörper

Antigen: Casein Kinase 1, epsilon (CSNK1E)

Synonyme: CKIe, hckie, CKIepsilon, ck1-epsilon, CSNK1E, Csnk1e, dco, MGC143296, fj38c01, MGC77310, zgc:77310, wu:fj38c01, wu:fj62a04, HCKIE, MGC10398, tau, AI426939, AI551861, AW457082, CK1epsilon, KC1epsilon

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391600

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: CK1 epsilon (CK1e) (C-term)

Gen-ID: 1454

Swiss-Prot: P49674

Immunogen: This CK1e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338~368 amino acids from the C-terminal region of human CK1e.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Casein kinase I, epsilon isoform, CKI-epsilon, CKIe

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CK1e.

Molekulargewicht: 47315 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-CK1e C-term Pab (ABIN391600) in SKBR3 cell lysate. CK1e (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Gao, Inuzuka, Tseng et al.: "Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction." in: Nature cell biology, Vol. 11, Issue 4, pp. 397-408, 2009 (PubMed).