O6-Methylguanine-DNA-Methyltransferase (MGMT) (C-Term) Antikörper

Antigen: O6-Methylguanine-DNA-Methyltransferase (MGMT)

Synonyme: MGMT

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391514

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: MGMT (C-term)

Gen-ID: 4255

Swiss-Prot: P16455

Immunogen: This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61~90 amino acids from the C-terminal region of human MGMT.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MGMT.

Molekulargewicht: 21646 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Signaltransduktion, Proteinmodifikationen, Krebs, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of MGMT antibody (C-term) (ABIN391514) in CEM cell line lysates (35ug/lane). MGMT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.