Phosphoglycerate Kinase 1 (PGK1) (Center) Antikörper

Antigen: Phosphoglycerate Kinase 1 (PGK1)

Synonyme: PGKA, MIG10, MGC8947, MGC117307, MGC142128, Pgk-1, MGC118097, Pgk, MGC105265, PGK1, PGK, zgc:56252, zgc:77899, wu:fd59b07, wu:fj36g06, MGC127284, PHOSPHOGLYCERATE KINASE 1

Epitope: Center

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391135

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: PGK1 (Center)

Gen-ID: 5230

Swiss-Prot: P00558

Immunogen: This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2, PGKA

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.

Molekulargewicht: 44483 DA

Anwendungen

Applikationshinweise: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometry

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Krebs, Zellzyklus, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of hPGK1-G132 (ABIN391135) in Hela, HepG2 cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.

Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132) (ABIN391135). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.

Subcellular fractionation of HEK-293T cells transiently transfected with myc-tagged WT or mutant LRRK2 and analyzed by Western blotting. Enrichment of each fraction was assessed by stripping the membrane and reprobing with the indicated marker antibody. Two micrograms of protein per lane was loaded, and LRRK2 expression was evaluated by using anti-myc antibody. (PNAS 2005 Nov 15.102(46):16842-16847)

Publikationen

Publikationen: Danshina, Geyer, Dai et al.: "Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice." in: Biology of reproduction, Vol. 82, Issue 1, pp. 136-45, 2009 (PubMed).