Deoxycytidine Kinase (DCK) (C-Term) Antikörper

Antigen: Deoxycytidine Kinase (DCK)

Synonyme: dck, MGC80405, DCK, Dck, dck2, MGC174279, wu:fe11h12, wu:fj05e11, zgc:109951, wu:fc15f06, zgc:101771, MGC127248, MGC117410, MGC138632

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391125

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: DCK (C-term)

Gen-ID: 1633

Swiss-Prot: P27707

Immunogen: This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the C-terminal region of human DCK.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Deoxycytidine kinase, dCK

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCK.

Molekulargewicht: 30518 DA

Anwendungen

Applikationshinweise: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 IP~~1:500~1:1000 Immunohistochemistry ~~ 1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Krebs, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The anti-DCK Pab (ABIN391125) is used in Western blot to detect DCK in mouse intestine tissue lysate.

Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (ABIN391125) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (ABIN391125). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.

DCK Antibody (C-term) (ABIN391125)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publikationen

Publikationen: Hartford, Duan, Delaney et al.: "Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity." in: Blood, Vol. 113, Issue 10, pp. 2145-53, 2009 (PubMed).