SMURF1 Antikörper (N-Term)

Produktnummer ABIN388828

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SMURF1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für SMURF1 Antikörper (N-Term) (ABIN388828)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMURF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB04101

Produktdetails anti-SMURF1 Antikörper

Bindungsspezifität: AA 66-96, N-Term

Homologie: X

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-SMURF1 Antikörper (ABIN388828)

He, Yang, Isales, Shi: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-? inhibition of mesenchymal stem cell osteogenic differentiation." in: PLoS ONE, Vol. 7, Issue 3, pp. e31717, (2012) (PubMed).

Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).

Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).

Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).

Details zu SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Andere Bezeichnung: SMURF1

Hintergrund: Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Molekulargewicht: 86114

Gen-ID: 57154

NCBI Accession: NP_001186776, NP_065162, NP_851994

UniProt: Q9HCE7

Pathways: Regulation of Cell Size