LC3 (APG8) Antikörper

Antigen: LC3 (APG8)

Klonalität: Monoklonal (166AT1234)

Wirt: Maus

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunfluoreszenz (IF), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN387792

Produktbeschreibung

Produktmerkmale: Purified Mouse Monoclonal Antibody (Mab)

Gen-ID: 84557

Swiss-Prot: Q9H492

Immunogen: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 166AT1234

Beschreibung: Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate

Spezifität: This antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Molekulargewicht: 14272 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:25~100 Immunocytochemistry ~~ 1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Zelluläre Signaltransduktion, Zellstruktur, Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

(TOP LEFT)Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract), Lane 2: 293 transfected with human LC3 (whole cell extract). (TOP RIGHT)Mouse cerebellar cell lines stably expressing human GFP-LC3 fusion protein were evaluated by immunocytochemistry using the anti-LC3 monoclonal antibody ABIN387792. Punctuate autophagic cell vesicles , with numerous GFP-LC3 vesicles are displayed. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization. (BOTTOM) Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. (TOP) 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# ABIN387792. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center. (MIDDLE) Cathepsin D WT vs KO (postnatal day 25) mouse thalamus exhibiting clumped immunoreactivity for LC3 in the KO brain (arrows, upper panel) and relative absence of clumped LC3 immunoreactivity in the WT control (staining is diffuse and cytoplasmic, lower panel). Data courtesy of Dr. John Shacka, Neuropathology Division, University of Alabama at Birmingham. (BOTTOM) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Autophagy LC3 (APG8) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-LC3 (APG8) antibody (Image 2)

Publikationen

Publikationen: Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).