PR Domain Containing 2, with ZNF Domain (PRDM2) Antikörper

Antigen: PR Domain Containing 2, with ZNF Domain (PRDM2)

Synonyme: RIZ, KMT8, RIZ1, RIZ2, MTB-ZF, HUMHOXY1, Riz, Riz1, Gm1033, Znfpr1c1, 4833427P12Rik, E330024L24Rik, MGC114763, riz, kmt8, riz1, riz2, mtb-zf, humhoxy1, PRDM2

Klonalität: Monoklonal (33AT1045)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN387783

Produktbeschreibung

Produktmerkmale: Purified Mouse Monoclonal Antibody (Mab)

Weitere Bezeichnung: PRDM2 (RIZ1)

Gen-ID: 7799

Immunogen: "Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2)."

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 33AT1045

Beschreibung: Other names: PR-domain zinc finger protein 2 (PRDM2) Retinoblastoma protein-interacting zinc-finger protein (RIZ1) (MTE-binding protein) (MTB-ZF)., KMT8

Spezifität: Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).

Molekulargewicht: 189011 DA

Anwendungen

Applikationshinweise: ELISA~~1:1,000 Western blotting~~1:100~500 Immuno precipitation

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Signaltransduktion, Proteinmodifikationen, Krebs, Chromatin, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-PRDM2 Mab (Cat. #ABIN387783) in lysate from transformed cells. Lane A: mock plasmid, Lane B: PRDM2[1-347]-encoding plasmid. PRDM2[1-347] (arrow) was detected using purified Mab. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibodies against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis.

anti-PR Domain Containing 2, with ZNF Domain (PRDM2) antibody (Image 2)

Publikationen

Publikationen: Lakshmikuttyamma, Takahashi, Pastural et al.: "RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression." in: Journal of hematology & oncology, Vol. 2, pp. 28, 2009 (PubMed).