BID Antikörper (C-Term)

Produktnummer ABIN374543

Der Ziege Polyklonal anti-BID Antikörper wird verwendet zum Nachweis von BID in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für BID Antikörper (C-Term) (ABIN374543)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-BID Antikörper

Bindungsspezifität: C-Term

Sequenz: C-TYVRSLARNG MD

Spezifität: This antibody reacts to BH3-interacting domain death agonist. It is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1. Genename: BID

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: BID

Hintergrund: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.Synonyms: BH3-interacting domain death agonist, p22 BID

Molekulargewicht: 26.8kDa (NP_932070.1) and 22.0kDa (NP_001187.1).

Gen-ID: 637, 9606

UniProt: P55957

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity