GRB2 Antikörper (C-Term)

Produktnummer ABIN374150

Der Ziege Polyklonal anti-GRB2 Antikörper wird verwendet zum Nachweis von GRB2 in Proben von Human, Maus, Ratte, Hund und Rind (Kuh). Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für GRB2 Antikörper (C-Term) (ABIN374150)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GRB2 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-PRNYVTPVNR NV

Spezifität: This antibody detects GRB2 / ASH at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Andere Bezeichnung: GRB2 / ASH

Hintergrund: Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2

Gen-ID: 2885

NCBI Accession: NP_002077

UniProt: P62993

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation